Table of Contents
How is chromatography used in fingerprinting?
Chromatographic fingerprinting is used for both establishing the identity and quality of the single herbs being added into herbal formulation . Nowadays chromatographic fingerprinting is used at two stages during the manufacture of a herbal formulation.
What is HPLC fingerprinting?
Biological chromatographic fingerprinting is a relatively new concept in the quality control of herbal samples. Originally it has been developed with the application of HPLC, and recently herbal samples’ biological profiles have been obtained by means of thin-layer chromatography (TLC).
What is phytochemical fingerprinting?
Conclusion Phytochemical fingerprinting is very important for authentication of herbal products . Fingerprints obtained from HPTLC and GC-MS analysis could be useful in authentication and identification of the drug . It can be used to characterise different constituents present in compounds.
Which of the following is used as a carrier gas in gas chromatography?
Explanation: Nitrogen has reduced sensitivity. It is still one of the commonly used carrier gas in gas chromatography.
How is chromatography similar to DNA fingerprinting?
It’s used to separate long molecules, like proteins, RNA and DNA from one another. Different organisms have different DNA sequences, so electrophoresis can be used to identify organisms and for DNA fingerprinting.
Can chromatography be used to find fingerprints?
Now, a paper published in the journal Clinical Chemistry — Rapid, Secure Drug Testing Using Fingerprint Development and Paper Spray Mass Spectrometry — has revealed another use for the humble chromatography paper that could leave drugs users sweating. …
What is TLC fingerprinting?
Thin layer chromatography (TLC) is the preliminary step to identify the phytochemical constituents in a sample. High performance thin layer chromatography (HPTLC) can provide an electronic image of the chromatographic fingerprint and a densitogram to detect the presence of marker compounds in a plant sample.
What are biological fingerprints?
= DNA fingerprinting is a laboratory technique used to establish a link between biological evidence and a suspect in a criminal investigation. A DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA profiles are a match, then the evidence came from that suspect.
Which method is used for making of herbal preparation?
The herbal preparations thus obtained include extracts, decoctions, tinctures, essential oils and others. The processes involved include extraction, distillation, fractionation, concentration, fermentation, or other chemical or biological methods.
Why is oxygen not used in gas chromatography?
Whenever gases is used in the chromatography process, there’s a potential for gas leaks, whether from the supply lines, storage tanks, or from the chromatograph itself. Nitrogen gas displaces oxygen. If nitrogen were to leak, air levels would become deficient of oxygen and employees could suffer health problems.
Why is helium used as a carrier gas in gas chromatography?
Many gas chromatography (GC) labs use helium as a carrier gas because it is faster than nitrogen and safer than hydrogen. The faster analysis times, lower cost, and unlimited availability of hydrogen make it the best chromatographic choice, but its flammability means implementation must be carefully considered.
How is chromatography used in DNA?
DNA-affinity chromatography has been used for the purification of DNA-binding proteins that control various cellular processes. Enzymatic synthesis of a double-stranded DNA-silica or Sepharose prevents modification of nucleotide bases.