Table of Contents
- 1 Which is better the wet mount method or the hanging drop method?
- 2 What is the advantage of observing a hanging drop compared to a wet mount preparation quizlet?
- 3 What is the advantage of the hanging drop procedure over the wet mount quizlet?
- 4 What are the advantages of a wet mount?
- 5 What is an advantage of using a wet mount preparation?
- 6 What are the other uses of the wet mount and hanging drop preparations?
- 7 Why should the drop be hanging?
- 8 How is phase contrast used in a microscope?
- 9 Where is the phase plate in a compound microscope?
- 10 How is phase shift detected in a microscope?
Which is better the wet mount method or the hanging drop method?
The wet mount tend to dry out quickly under the heat of the microscope light; it is simpler to perform than the wet mount, but it is useful for short-term observation only. The hanging drop is a more complex technique, but it allows for longer-term obervation and more reliable observation of motility.
What is the advantage of observing a hanging drop compared to a wet mount preparation quizlet?
What is the advantage of observing a hanging drop, compared to a wet mount preparation? Motility and larger organisms are better viewed in a hanging drop due to the greater depth. When viewing an unstained, live specimen, how do you distinguish between true motility and Brownian movement?
What type of microscopy is used for a hanging drop slide?
Light microscopy
Light microscopy The microscope is the tool that allows them to see the organisms that they study because of the magnifying power of the lenses.
What is the advantage of the hanging drop procedure over the wet mount quizlet?
What is the advantage of the hanging-drop procedure over the wet mount? The concave center will decrease the chances of Brownian motion. Why is petroleum jelly used in the hanging drop procedure? Why are microorganisms hard to see in wet preparations?
What are the advantages of a wet mount?
Advantages and disadvantage of a wet mount Compared to permanently mounted slides, wet mounts do have certain advantages: Quick preparation: specimen fixation, dehydration and staining are not necessary (but possible, if required). For this reason, wet mounts are the first kind of mounts that students learn to make.
What is one advantage of using hanging drop rather than wet mount?
Advantages: Like the wet mount, the hanging drop method preserves cell shape and arrangement. The Vaseline-sealed depression also slows down the drying-out process, so the organisms can be observed for longer periods. Disadvantages: The hanging drop method is also far too risky to use with highly pathogenic organisms.
What is an advantage of using a wet mount preparation?
Compared to permanently mounted slides, wet mounts do have certain advantages: Quick preparation: specimen fixation, dehydration and staining are not necessary (but possible, if required). For this reason, wet mounts are the first kind of mounts that students learn to make.
What are the other uses of the wet mount and hanging drop preparations?
Observing living microorganisms: To observe living microorganisms they must be kept in a liquid environment. Both wet mounts and hanging drop preparations can be used to observe living microbes.
Why are microorganisms hard to see in wet mount?
Why are microorganisms hard to see in wet preparations? Why isn’t the oil immersion lens used in the hanging-drop procedure? Since the hanging-drop procedure has a different refraction index, the oil immersion lens cannot refract the light correctly, due to its incompatibility.
Why should the drop be hanging?
It is an important tool to study the bacterial motility along with the shape, size and arrangement of the bacteria. It does not distort the cell shape and arrangement. Besides, the hanging drop method provides a better view of motility in bacteria than the wet mount method.
How is phase contrast used in a microscope?
This makes the cells barely, or not at all, visible in a brightfield microscope. Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in the light passing through a transparent specimen to brightness changes in the image.
Where is the annular groove in phase contrast microscopy?
The annular diaphragm. It is situated below the condenser. It is made up of a circular disc having a circular annular groove. The light rays are allowed to pass through the annular groove. Through the annular groove of the annular diaphragm, the light rays fall on the specimen or object to be studied.
Where is the phase plate in a compound microscope?
A compound microscope equipped for negative phase contrast has two additional components: a “phase plate” that retards light exactly 1⁄4wavelengthin a centered, ring-shaped area located at the back focal plane of the objective lens and a matching “phase annulus” in the condenser consisting of a clear ring on a black field (Figure 2-3B).
How is phase shift detected in a microscope?
The phase shift is not detectable by the eye so the resulting image on the image plane in the microscope appears as a normal bright background. Conversely, light that passes through the specimen may be diffracted by edges and local irregularities within the tissue.